Fig. 4.

Involvement of ERK/CREB signaling in the cytoprotective effect of Artemisinin. (A) D407 cells were pre-treated with 30 µM Artemisinin. The cells were collected at 0, 30, 60, and 120 min. The expression of phosphorylated ERK1/2, total ERK1/2, phosphorylated Akt, total Akt and phosphorylated CREB, total CREB and beta-actin were detected by Western blotting with specific antibodies (A). (B)(C)(D) Quantification of representative protein band from Western blotting. **P<0.01,***P <0.005 versus the 0 min group was considered significantly different.