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In cis and in trans modification crosstalk imposed by H3K4me on H3T3 phosphorylation activity of Haspin. A) Schematic illustration of asymmetrically methylated on H3K4 nucleosomes reacted with Haspin kinase enables H3T3ph mapping, in cis and in trans. B) 1H/15N SOFAST-HMQC and 1H/13C HSQC spectra (selected regions) of asNucs before and after reaction with Haspin kinase. C) Time-resolved NMR readouts of H3T3ph by Haspin on H3K4me asNucs
