Figure 1.

Gating strategy used to identify T cells and NK cells by flow cytometry. Lymphocytes were gated based on side scatter (SSC) and forward scatter (FSC) properties. Live cells (negatively stained for the Live/Dead-marker) were gated either as CD3⁻CD56⁺ cells (NK cells) or as CD3⁺CD56⁻ cells, which were further divided as CD4⁺ T cells, CD8⁺ T cells, pan γδ TCR⁺ cells (γδ T cells), or CD161⁺Vα7.2⁺ cells (MAIT cells). For all figures, gating strategies are described in the section “Materials and Methods.”