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. 2016 Jul 11;7:273. doi: 10.3389/fimmu.2016.00273

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Copyright © 2016 Johansson, Björkander, Mata Forsberg, Qazi, Salvany Celades, Bittmann, Eberl and Sverremark-Ekström.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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S. aureus and SEA-induced IFN-γ responses are partly IL-12-dependent. PBMC were cultured in the presence of an IL-12 neutralizing or isotype antibody, stimulated as indicated, and analyzed by flow cytometry. The relative percentage of IFN-γ⁺ cells among the CD4⁺ (n = 7), CD8⁺ (n = 7), γδ TCR⁺ (n = 7), CD161⁺Vα7.2⁺ (n = 4), and CD3⁻CD56⁺ (n = 7) lymphocyte populations is shown. The percentage of IFN-γ⁺ cells in isotype-treated PBMC stimulated with S. aureus or SEA is set to 1, and the percentage of IFN-γ⁺ cells in α-IL-12-treated cultures is relative to the isotype. Bars show medians with interquartile range.