Figure 3.

Acquisition of a Polyploid Giant-Cell Phenotype in the CSL-Deficient Cells

(A) Brightfield images of CSL^+/+ and CSL^−/− cells (upper panel) and high-magnification view of CSL^+/+ and CSL^−/− cells stained with DAPI and β-tubulin (lower panel). Giant cells are marked with arrows and polyploid cells are marked with arrowheads.

(B) Proportion of cells without mitotic exit division and cells generating several daughter cells among CSL^+/+ and CSL^−/− cells. At least 50 cells of each clone were analyzed.

(C) Time-lapse images of control and CSL-deficient cells. The white arrowheads denote cells analyzed by time-lapse imaging.

Scale bars: 100 μm (A upper), 50 μm (A lower), and 50 μm (C).