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. 2016 Jul 11;6:29601. doi: 10.1038/srep29601

Figure 2. The role of neutrophil- or monocyte-mediated inflammation in myocardial IR injury.

Figure 2

(A,B) The flow cytometric analysis demonstrates that pretreatment with an anti-Ly6G 1A8 monoclonal antibody decreased the number of neutrophils in the peripheral blood and the heart after 12 hours of reperfusion (N = 4 per group). (C) Pretreatment with anti-Ly6G antibody did not reduce infarct size 24 hours after reperfusion (N = 8 mice per group). (D,E) The number of monocytes in the blood and the heart after 12 hours of reperfusion was significantly reduced in CCR2−/− mice compared with CCR2+/+ mice (N = 5 mice per group). (F) The time series of infarct size assessment after reperfusion of various durations (3 h, 12 h, 24 h after IR) on WT mice and CCR2−/− mice (N = 6–8 mice per group). Data are expressed as the mean ± SD. Data are compared using the unpaired t-test. All results are average of three replicates. NS: not significant. SSC: side scatter, FSC: forward scatter.