Table 1.
Modification percentages for individual residues of β2m before and after heating at 75 °C for 24 hours or oxidation with 3% H2O2 for 24 hours.
| β2m | Native (control) |
Heated for 1 day |
Significant* | 3% H2O2 | Significant* |
|---|---|---|---|---|---|
| N-term | 49 ± 1 | 44 ± 2 | yes | 7.6 ± 0.3 | yes |
| Lys6 | 2.5 ± 0.1 | 3 ± 0.5 | no | 0 ± 0 | yes |
| Ser11 | 20 ± 3 | 11.8 ± 0.3 | yes | 6 ± 2 | yes |
| His13 | 32 ± 2 | 17 ± 1 | yes | 4 ± 1 | yes |
| Lys19 | 2.7 ± 0.4 | 2 ± 2 | no | 0 ± 0 | yes |
| Ser20 | 1.1 ± 0.3 | 4.8 ± 0.7 | yes | 1.7 ± 0.3 | no |
| Ser28 | 0.25 ± 0.05 | 0 ± 0 | yes | 0.3 ± 0.09 | yes |
| His31 | 1.9 ± 0.2 | 0.72 ± 0.08 | yes | 0 ± 0.1 | yes |
| Ser33 | 0.68 ± 0.09 | 0 ± 0 | yes | 0.18 ± 0.01 | yes |
| Lys41 | 0.6 ± 0.1 | 1 ± 0.1 | yes | 0 ± 0 | yes |
| Lys48 | 0 ± 0 | 1.2 ± 0.2 | yes | 0 ± 0 | no |
| His51 | 2.2 ± 0.3 | 0 ± 0 | yes | 0.27 ± 0.07 | yes |
| Ser55 | 1.4 ± 0.2 | 0 ± 0 | yes | 0 ± 0 | yes |
| Ser57/ Lys58 |
2.5 ± 0.4 | 0 ± 0 | yes | 0 ± 0 | yes |
| Tyr67/ Thr68 |
2.2 ± 0.3 | 2 ± 1 | no | 6 ± 2 | yes |
| Lys91 | 2.1 ± 0.4 | 2 ± 0.2 | no | 0.1 ± 0.2 | yes |
| Lys94 | 3.1 ± 0.6 | 3 ± 0.4 | no | 0.2 ± 0.3 | yes |
*
A difference was considered significant if the p-value, calculated by performing an unpaired T-test, was less than 0.05 (corresponding to a 95% confidence level, n=3)).