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. Author manuscript; available in PMC: 2017 Apr 5.
Published in final edited form as: Anal Chem. 2016 Mar 24;88(7):3967–3975. doi: 10.1021/acs.analchem.6b00191

Figure 1.

Figure 1

Overview of the SEP discovery workflow. To identify known and novel SEPs MS/MS spectra are searched against the Human UNIPROT database (known SEPs) and a 3-frame translated RNA-Seq custom database (novel SEPs). Peptides that uniquely match to a UNIPROT protein entry that is less than 150 amino acids in length are annotated as known SEPs. Peptides that match to an entry in the form RNA-Seq 3-frame translated database that is less than 150 amino acids in length and do not overlap with any UNIPROT proteins are novel SEPs (i.e. non-annotated, non-UNIPROT).