Skip to main content
. Author manuscript; available in PMC: 2017 Apr 5.
Published in final edited form as: Anal Chem. 2016 Mar 24;88(7):3967–3975. doi: 10.1021/acs.analchem.6b00191

Figure 6.

Figure 6

Some novel SEPs are new isoforms of known proteins or fragments of longer proteins. (A) SEP252 is a new isoform of the protein alpha-endosulfine (ENSA) and this connection were discovered because the SEP peptide (red) is homologous to ENSA but different enough to realize that this peptide is from a non-annotated smORF. Alignment of the entire smORF demonstrates high sequence homology (>80%) to various ENSA isoforms indicating that this SEP is a member of the ENSA family of proteins. (B) SEP266 was identified through a peptide that is homologous (red) to another peptide from Elongation factor 1-alpha 1 (EEF1A1) but differs by one amino acid (blue) indicating that it belongs to a non-annotated smORF. Alignment of the entire smORF shows high sequence homology (>80%) to part of EEF1A1.