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. 2016 Jul 11;16:140. doi: 10.1186/s12866-016-0721-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Relationship between AT content and normalized predicted free energy of 16S rRNA, showing the grouping of sequences by bacterial transmission mode. Free energy estimation was constrained to known secondary structure interactions (in the E. coli rRNA molecule) and calculated using the Turner 2004 model. Analysis was based on the region corresponding to E. coli coordinates 16–1477. Shape indicates transmission mode (when known), and colors indicate bacterial lifestyle. AAB1 and AAB2 from C. chromaiodes are marked (blue stars; extracellular gut associates of unknown transmission mode). The closely related AAB lineage from Atta laevigata appears as a yellow star (unknown lifestyle and unknown transmission mode). The names and values for all points are provided in Additional file 7. Lifestyle designations are with respect to insect hosts