Table 5.

Yield of heterodimer protein peak from the Ni-NTA column in the presence of various synthetic GST pi peptides^a

GST pi peptides	Peak II (heterodimer)
	Total Abs280 nm units	Total GST activity unitsb (ΔOD340 nm/min)
1. None	1.18	1.36
2. Residues 41-85	No peak	No peak
3. Residues 115-124	No peak	No peak
4. Residues 131-163	0.49	0.62
5. Residues164-197	0.66	0.74
6. Residues 32-40	1.10	1.21
7. Residues 86-97	0.94	1.09

^aGST pi and 1-Cys Prx proteins (40 μM of each) together with 100 μM of synthetic GST pi peptide were treated with 20% 1,6-hexanediol followed by dialysis against buffer containing 2.5 mM GSH and loaded onto a Ni-NTA column, as described in Materials and methods.

^bGST activity was measured using CDNB as the electrophilic substrate, as described in Materials and methods, and expressed as ΔOD_{340 nm}/min.