Skip to main content
. 2016 Apr 28;6(5):772–783. doi: 10.1016/j.stemcr.2016.04.002

Figure 6.

Figure 6

Epigenetic Modifications of SOX2 Promoter Leading to RA-Induced Derepression Are Modulated by UTX

(A) Expression of SOX2, POU5F1, and NANOG in the RA-treated UTX-KD cells compared with scramble (scr) cells in a 6-day time-course experiment.

(B) qRT-PCR showing expression level of SOX2, NANOG, POU5F1, and UTX in the untreated scr versus UTX-KD cells and treated scr versus UTX-KD cells. Data represent mean ± SD of the three independent experiments. ∗∗p < 0.01 between treated UTX-KD and scr by Student’s t test.

(C) ChIP-qPCR showing loss of H3K27me3 enrichment at SOX2 promoter of RA-treated scr cells compared with untreated cells, whereas the enrichment is unchanged in RA-treated UTX-KD cells. Data represent mean ± SD of three independent experiments. Student’s t test: ns, not significant; ∗∗p < 0.01; ∗p < 0.05.

(D) ChIP-qPCR with H3K4me3 antibody showing enrichment at SOX2 promoter of RA-treated scr cells compared with untreated scr cells and RA-treated UTX-KD cells compared with untreated KD cells. Data represent mean ± SD of three independent experiments. Student’s t test: ns, not significant; ∗∗p < 0.01; ∗p < 0.05.