Figure 7.

Neuronal Marker Genes Are Upregulated following SOX2 Derepression in TCam-2 Cells in Response to Retinoid Signaling

(A) Representative IF showing staining with (a) neurofilament (NEFM), (b) nestin (NES), (c) neuron-specific class III β-tubulin (TUJI), and (d) neurotrophic tyrosine kinase receptor type 3 (TRKC). Left image, DAPI; middle image, neuronal gene; right image, merged. For each antibody the upper panel represents untreated cells and the lower panel RA-treated cells. Scale bars represent 100 μm.

(B) Expression of neuronal genes in RA-treated UTX-KD cells compared with RA-treated scramble cells by qRT-PCR. Data represent mean ± SD of three independent experiments. ^∗∗ p < 0.01, ^∗p < 0.05 by Student’s t test.

(C) GO categories enriched in ARACNe-predicted SOX2 targets of TCam-2 cells following RA treatment. Blue bar represents actual enriched number of genes; red bar represents expected enrichment by chance.