Fig. 2.

Inhibition of osteoclast differentiation in BMMs by donepezil. A, Effect of donepezil (0, 0.5 and 5 μg/ml) on osteoclast differentiation of BMMs: (upper panel) TRAP staining, (lower panel) number of TRAP-positive multinucleated cells formed (Scale bar, 1.0 mm). Data are calculated from four repeated experiments. B, mRNA expression of osteoclast-specific genes in BMMs treated with RANKL with or without donepezil (0, 0.5 and 5 μg/ml) for 5 days estimated by qPCR analysis: (left panel) NFATc1, (right panel) cathepsin K . Data are calculated from three repeated experiments. #, p < 0.05; ##, p < 0.01. Data are expressed as the means ± S.D.