Figure 3.

Proliferation of Zfyve27-CreERT2-Marked Cells during Kidney Repair from Injury

(A) Fraction (%) of tdTomato⁻ cells (identified with DAPI) and of tdTomato⁺ cells (i.e., Zfyve27-CreERT2-marked cells) that incorporated EdU at 3, 8, and 21 days after KI. EdU incorporation in tdTomato⁻ cells 21 days after KI was significantly lower than at 3 and 8 days (p < 0.05) and, as shown in the figure, than that of the tdTomato⁺ cells at this time (p < 0.05). n = 3–5 mice, 8 days pTM. Mean ± SE.

(B) EdU incorporation in the upper papilla of a Zfyve27-CreERT2;R-tdTomato mouse 3 days after KI. Scale bar, 40 μm.

(C) Cluster of tdTomato⁺ cells in the upper papilla that incorporated EdU 8 days after KI. Scale bar, 40 μm.

(D) Tubules made up of tdTomato⁺ cells that incorporated EdU 21 days after KI. Scale bar, 50 μm.