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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Biochemistry. 2015 Apr 28;54(18):2851–2857. doi: 10.1021/bi501540c

Figure 2.

Figure 2

Steady state kinetics for PHD2 variants with varied [2OG]. Reaction mixtures included (NH4)2Fe(SO4)2 (10 µM), ascorbic acid (1 mM), 2OG (1–3000 µM), and CODD (10 µM) in 50 mM HEPES pH 7.00, 37.0 °C, ambient [O2] (217 µM).