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Steady state kinetics as a function of varied [O2]. Reaction mixtures included (NH4)2Fe(SO4)2 (10 µM), 2OG (100 µM), CODD (10 µM), and ascorbic acid (1 mM) in 50 mM HEPES pH 7.00. (open circles) WT-PHD2, (closed circles) Thr387→Asn, (triangles) Thr387→Ala.
