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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Biochemistry. 2015 Apr 28;54(18):2851–2857. doi: 10.1021/bi501540c

Table 2.

Steady state kinetic constants, kcat and kcat/KM(CODD) with varied [CODD].a

Enzyme kcat, min−1 kcat/KM(CODD), µM−1min−1 KI, µM KM, µM
WT-PHD2 1.9 ± 0.1 2.4 ± 0.2 - 0.8 ± 0.1
Thr387→Ala 44 ± 3 11 ± 1 38 ± 6 4.1 ± 0.7
Thr387→Asn 0.9 ± 0.1 0.7 ± 0.1 - 1.4 ± 0.2
a

. Reactions contained (NH4)2Fe(SO4)2 (10 µM), ascorbic acid (1 mM), 2OG (100 µM), and CODD (2 – 50 µM) in 50 mM HEPES pH 7.00, 37.0 °C, ambient [O2](217 µM).