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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Gene Ther. 2015 Aug 23;23(1):78–85. doi: 10.1038/gt.2015.78

Figure 1.

Figure 1

Validate miRNA-mediated PPARδ knockdown. (a) C2C12 cells were infected with the lentiviruses expressing control and PPARδ miRNA (sequence A). After 48 h, the cells were collected and expression of endogenous PPARδ was determined using western blot analysis. (b) Specific PPARδ knockdown. BMDM were infected with the indicated miRNA and the expression levels of PPAR δ, α and γ were determined using western blot analysis (In a and b, the numbers indicate the relative expression of the indicated proteins after normalization to β-actin). (c) The expression of endogenous PPARδ in BMDM infected with indicated viruses was determined by real-time PCR. The mRNA levels in BMDM infected with Lenti-PPARδ-miRNA were normalized to those in BMDM infected with Lenti-Ctl-miRNA (**P<0.01).