Figure 5.
PPARδ knockdown represses expression of IL-6 and IL-1β in macrophages. The relative expression of IL-6 in elicited peritoneal macrophages (a) as well as IL-6 and IL-1β transcripts in BMDM treated with GW 501516 was determined using quantitative PCR (b and c, respectively) as indicated (open bars: Lenti-Ctl-miRNA, solid bars: Lenti-PPARδ-miRNA). BMDMs were subjected to incubation in Dulbecco’s modified Eagle’s medium supplemented with 10% lipoprotein-deficient serum (LPDS) overnight, followed by GW501516 (0.1 μM) treatment for 24 h. (*P<0.05 and **P<0.01).