Table 1.
Primer sequences for real-time PCR
| Genes | Access number | Primer sequences (5–3′) | Temperature (°C) | Product size |
|---|---|---|---|---|
| β-Actin | NM_007393 | F: TTGCTGACAGGATGCAGAAGGAGA | 59.9 | 159 bp |
| R: ACTCCTGCT TGC TGATCCACATCT | 60.0 | |||
| PPARδ | NM_011145 | F: GCCCAAGTTCGAGTTTGCTGTCAA | 59.9 | 161 bp |
| R: ATTCTAGAGCCCGCAGAATGGTGT | 60.0 | |||
| ABCA1 | AF287263.1 | F: CCCTGCTACACTGGTCATTATC | 61.0 | 103 bp |
| R: CCCATACAGCAAGAGCAGAA | 60.9 | |||
| CCR2 | MMU56819 | F: AATGAGAAGAAGAGGCACAGGGCT | 60.0 | 161 bp |
| R: ATGGCCTGGTCTAAGTGCTTGTCA | 60.2 | |||
| MCP-1 | NM_011333 | F: AGCAGGTGTCCCAAAGAAGCTGTA | 60.1 | 176 bp |
| R: TGAAGACCTTAGGGCAGATGCAGT | 60.2 | |||
| MMP-9 | NM_013599 | F: CCATGGCAAATTCTTCTGGCGTGT | 60.1 | 162 bp |
| R: TAGAGACTTGCACTGCACGGTTGA; | 60.0 | |||
| IL6 | DQ788722 | F:ATCCAGTTGCCTTCTTGGGACTGA | 60.0 | 134 bp |
| R:TAAGCCTCCGACTTGTGAAGTGGT | 59.8 | |||
| TNFα | Y00467 | F:AGCCGTGACTGTAATTGCCCTACA | 60.1 | 115 bp |
| R:TTTAGGCCTCCGCAAAGAGATGGA | 60.0 | |||
| ADRP | M93275 | F:ACCTTGTGTCCTCCGCTTATGTCA | 59.8 | 170 bp |
| R:GGCATAGGTATTGGCAACCGCAAT | 59.9 |