Figure 1.

Gene expression analysis supports the cell identity and homogeneity of the WA09-human embryonic stem cell (hESC) → ISL1+ nascent mesoderm (INM) → smooth muscle cell (SMC) differentiation. (A) The principle component analysis (PCA) was performed with the entire transcript set (22,089 in total) included in the Affymetrix Human Gene 1.0 ST array. (B) Heat map indicates genes with significant changes (red: upregulated; green: downregulated) among the 3 cell types and their enriched functional groups (see also Table S1).