Table 2.

Differences in mean HDL-carried has-miR-223-3p and has-miR-135a-3p relative concentrations between the 3 experimental diets (n = 9).¹¹

	HDL-miR-223-3p concentrations			HDL-miR-135a-3p concentrations
Group comparisons	Ct ratio2	Fold change	Friedman's test P value (and Padj value)3	Ct ratio2	Fold change	Friedman's test P value (and Padj value)3
Control4	0.830 ± 0.055	1.046	0.459 (0.918)	0.621 ± 0.014	1.357	0.459 (0.918)
rTFA	0.830 ± 0.041			0.627 ± 0.030
Control4	0.830 ± 0.055	−1.218		0.621 ± 0.014	1.094
iTFA	0.819 ± 0.045			0.621 ± 0.018
rTFA4	0.830 ± 0.041	−1.274		0.627 ± 0.030	−1.241
iTFA	0.819 ± 0.045			0.621 ± 0.018

¹Control: diet low in TFA from any source; Ct: cycle threshold; P_adj: Bonferroni adjusted P-value for multiple testing; SD: standard deviation; iTFA: diet rich in TFA from industrial source; rTFA: diet rich in TFA from ruminant; TFA: trans fatty acids.

²HDL-carried miRNA relative concentration or Ct ratio = miR-223-3p (or miR-135a-3p)/cel-miR-39 cycle threshold (Ct) ratio (1/x) values. Mean ± Standard deviation.

³Differences in HDL-carried miRNA relative concentrations between the 3 diets were assessed using the Friedman's test, with Bonferroni adjusted P values for multiple testing. Adjusted P value = Friedman's test P value multiplied by 2 independent tests performed (2 miRNAs tested). Means were considered significantly different between diets at P_adj ≤ 0.05.

⁴Group to which the data are compared for the fold change calculation for each diet pair.