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. 2016 May 13;11(7):539–552. doi: 10.1080/15592294.2016.1184800

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 9. — MeDIP DNA methylation analysis of Fitm1 gene. (A) Genomic organization of Fitm1 exon 1, putative transcription start site (TSS), and position of identified CpG island. Amplified sequence is shown in detail (primer binding sites in bold letters and analyzed CpG dinucleotides bold / underlined, TSS indicated with +1), (B) degree of DNA methylation of Fitm1 exon 1 in liver tissue of wt offspring (empty bar, wt father / wt mother; gray bar, wt father / eNOS^+/− mother; $ P < 0.05 vs. wt father / wt mother).