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. 2016 May 13;11(7):539–552. doi: 10.1080/15592294.2016.1184800

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 10. — MeDIP DNA methylation analysis of Cdkn1a gene. (A) Genomic organization of the Cdkn1a gene showing first exons, transcription start site (TSS), and the position of identified CpG islands; amplified sequence of CpG island 4 is shown in detail (primer binding sites in bold letters and analyzed CpG dinucleotides bold / underlined). (B) Degree of DNA methylation of CpG island 4 in liver tissue of wt offspring (empty bar, wt father / wt mother; gray bar, wt father / eNOS^+/− mother;# P < 0.01 vs. wt father / wt mother).