Table 2.
Amino acid concentration in offspring liver tissue.
| Offspring of both sexes |
Male offspring |
Female offspring |
||||
|---|---|---|---|---|---|---|
| WT (F:WT; M:WT) | WT (F:WT; M:eNOS KO) | WT (F:WT; M:WT) | WT (F:WT; M:eNOS KO) | WT (F:WT; M:WT) | WT (F:WT; M:eNOS KO) | |
| N | 48-50 | 30-36 | 21-22 | 15-18 | 27-28 | 15-18 |
| Alanine | 38.26 ± 1.42 | 37.37 ± 1.70 | 35.89 ± 1.63 | 34.57 ± 2.72 | 40.12 ± 2.14 | 40.18 ± 1.88 |
| Glycine | 19.97 ± 0.45 | 18.73 ± 0.63 | 21.02 ± 0.64 | 19.25 ± 0.81 | 19.15 ± 0.59 | 18.12 ± 0.96 |
| Urea | 18.77 ± 0.64 | 18.23 ± 0.69 | 19.28 ± 1.05 | 18.93 ± 0.81 | 18.37 ± 0.79 | 17.53 ± 1.13 |
| Valine | 3.50 ± 0.11 | 3.07 ± 0.12 + | 3.62 ± 0.13 | 2.84 ± 0.15 + | 3.41 ± 0.17 | 3.31 ± 0.18 |
| Leucine | 4.24 ± 0.14 | 3.65 ± 0.19 + | 4.57 ± 0.20 | 3.26 ± 0.20 + | 3.97 ± 0.17 | 4.03 ± 0.29 |
| Isoleucine | 1.90 ± 0.07 | 1.59 ± 0.07 + | 2.02 ± 0.08 | 1.44 ± 0.08 + | 1.80 ± 0.10 | 1.74 ± 0.10 |
| Proline | 2.92 ± 0.16 | 2.70 ± 0.16 | 2.72 ± 0.15 | 2.54 ± 0.24 | 3.07 ± 0.27 | 2.86 ± 0.22 |
| Methionine | 1.31 ± 0.04 | 1.14 ± 0.06 # | 1.36 ± 0.07 | 0.99 ± 0.06 + | 1.28 ± 0.05 | 1.29 ± 0.09 |
| Serine | 4.89 ± 0.19 | 4.42 ± 0.20 $ | 5.00 ± 0.26 | 4.14 ± 0.25 $ | 4.81 ± 0.27 | 4.72 ± 0.30 |
| Threonine | 3.11 ± 0.08 | 2.69 ± 0.13 + | 3.31 ± 0.13 | 2.40 ± 0.14 + | 2.96 ± 0.09 | 2.98 ± 0.19 |
| Phenylalanine | 1.70 ± 0.05 | 1.45 ± 0.08 + | 1.82 ± 0.09 | 1.26 ± 0.08 + | 1.61 ± 0.06 | 1.64 ± 0.13 |
| Aspartic acid | 3.16 ± 0.19 | 2.92 ± 0.11 | 2.97 ± 0.11 | 2.82 ± 0.16 | 3.30 ± 0.33 | 3.04 ± 0.14 |
| Cysteine | 0.26 ± 0.01 | 0.25 ± 0.03 # | 0.26 ± 0.01 | 0.20 ± 0.01 + | 0.25 ± 0.01 | 0.30 ± 0.07 |
| Glutamic acid | 8.63 ± 0.47 | 8.59 ± 0.51 | 8.13 ± 0.38 | 8.06 ± 0.72 | 9.02 ± 0.78 | 9.12 ± 0.71 |
| Lysine | 7.16 ± 0.35 | 6.38 ± 0.38 | 6.96 ± 0.50 | 5.56 ± 0.47 | 7.32 ± 0.49 | 7.19 ± 0.53 |
| Glutamine | 23.46 ± 1.16 | 20.16 ± 1.00 | 25.65 ± 1.40 | 21.68 ± 1.34 | 21.74 ± 1.72 | 19.55 ± 1.48 |
| Arginine | 1.22 ± 0.06 | 1.25 ± 0.11 | 1.07 ± 0.06 | 1.01 ± 0.09 | 1.35 ± 0.10 | 1.48 ± 0.19 |
| Histidine | 4.50 ± 0.13 | 3.78 ± 0.14 + | 4.84 ± 0.17 | 3.71 ± 0.23 + | 4.23 ± 0.18 | 3.84 ± 0.17 |
| Tyrosine | 1.82 ± 0.05 | 1.60 ± 0.07 # | 1.92 ± 0.08 | 1.40 ± 0.08 + | 1.75 ± 0.06 | 1.80 ± 0.10 |
| Tryptophan | 0.50 ± 0.01 | 0.41 ± 0.02 + | 0.49 ± 0.01 | 0.35 ± 0.02 + | 0.51 ± 0.02 | 0.47 ± 0.02 |
Concentration of aminoacids expressed as nmol/mg protein. F: Father, M: Mother, WT: Wild type, eNOS KO: heterozygous eNOS knockout, $ P < 0.05 vs. WT (F:WT;M:WT), # P < 0.01 vs. WT (F:WT;M:WT), + P < 0.001 vs. WT (F:WT;M:WT)