Table 2. Comparison of transcript fold change as detected by RNA-Seq and qPCR.
| Transcript Seq. ID | Seq. description | FPKMR line | FPKMS line | log2-fold | qPCR log2-folda | validated |
|---|---|---|---|---|---|---|
| 21345 | Rg1 | 15.72 | 0.79 | 4.29 | 3.76 ± 0.526 | yes |
| 10552 | Rg2 | 37.12 | 10.76 | 1.78 | 1.61 ± 0.368 | yes |
| 48569 | Rg3 | 20.39 | 2.97 | 2.77 | 3.68 ± 0.328 | yes |
| 26003 | Rg4 | 16.09 | 0.0 | - | 0.65 ± 0.201 | yes |
| 26004 | Rg5 | 15.78 | 0.0 | - | 2.3 ± 0.312 | yes |
| 26005 | Rg6 | 13.67 | 0.0 | - | 4.89 ± 0.472 | yes |
| 27596 | Rg7 | 14.38 | 1.56 | 3.19 | 4.63 ± 0.853 | yes |
| 31761 | Rg8 | 12.70 | 4.05 | 1.64 | 1.48 ± 0.345 | yes |
| 38700 | Rg9 | 12.65 | 0.0 | - | 2.75 ± 0.209 | yes |
| 40213 | Rg10 | 11.06 | 0.0 | - | Only in R line | yes |
| 40214 | Rg11 | 11.86 | 0.0 | - | 2.74 ± 0.671 | yes |
| 40215 | Rg12 | 13.21 | 0.0 | - | 6.81 ± 0.263 | yes |
| 18520 | PR sth-2-like | 170.77 | 3.42 | 5.63 | 8.59 ± 0.364 | yes |
| 39531 | PR leaf protein 4-like | 3045.13 | 5.85 | 9.02 | 11.76 ± 0.206 | yes |
| 22353 | LOX | 2132.67 | 3.02 | 9.46 | 11.30 ± 0.081 | yes |
aqPCR fold changes represent mean of three biological replicates.