Fig 3.

Western blot analysis of virion-associated Env content in cell-free supernatants harvested at six (a) and nine (b) days post-infection. The name of each SHIV being tested is indicated above each lane. Color-coding indicates whether the SHIVs has been adapted by macaque-passage (orange), in lab-culture (red), or represents a circulating HIV-1 variant (blue). For each sample, 5 ng of SIV p27 was loaded into each lane. The Env:p27 signal relative to SHIV AD8-EO indicated at the bottom of the panel for each virus is the average for two independent experiments. (c) Correlation between the slope of viral replication in the first 6 days of infection and the average virion-associated HIV-1 Env content of cell-free virions from 6 dpi was determined. (d) Correlation between average virion-associated Env content at 6 dpi and IFNα sensitivity as measured by AUC ratio (IFN+/IFN-). Spearman r represents the Spearman rank correlation coefficient.