Table 1. Differences in mitochondrial transcript abundance, polyadenylation and the extent of RNA editing in two life cycle stages of T. brucei.
| No. of uridines | Relative level of mature RNA | PolyA tail length | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene | Added | Deleted | Edited size (nt) | Stage Edited | Long Slender | Short Stumpy | Procyclic | Bloodstream | Procyclic | Number of PC major classesg | References |
| 12S | 2–17 (tail) | 0 | 1149 | N.D. | 0.04 | 1.3 | 1.0 | N.A. | N.A. | N.A.j | [9,21,22] |
| 9S | 7 (tail) | 0 | 611 | N.D. | 0.07 | 1.4 | 1.0 | N.A. | N.A. | N.A. | [9,21,22] |
| CYb | 34 | 0 | 1,151 | Pa | ~0 | 0.5 | 1.0 | Short (UEh & Ei) | Short (E) & Long (E) | 11 | [9,10,23,24] |
| A6 | 448 | 28 | 821 | P/BSb | 1.0 | N.D.f | 1.0 | Short (E) & Long (E) | Short (E) & Long (E) | 81 | [16,23] |
| COI | 0 | 0 | 1,647 | NEc | 0.07 | 0.4 | 1.0 | Short | Short & Long | N.A. | [9,24,25] |
| COII | 4 | 0 | 663 | P | ~0 | 0.5 | 1.0 | Short | Short (UE) & Long (E) | N.A.k | [9,24,26] |
| COIII | 547 | 41 | 969 | P/BS | 1.0 | N.D. | 1.0 | Short (E) | Short(E) & Long(E) | 151 | [17] |
| ND1 | 0 | 0 | 960 | NE | ~1 | N.D. | ~1 | Short & Long | Short & Long | N.A. | [25,27,28] |
| ND2 | 0 | 0 | 1343 | NE | >1.0 | N.D. | 1.0 | Short & Long | Short & Long | N.A. | [24,29,30] |
| ND3 | 210 | 13 | 452 | P/BS | >1.0 | N.D. | 1.0 | Short (E) | Short (E) | 34 | [11] |
| ND4 | 0 | 0 | 1314 | NE | ~1.0 | N.D. | ~1.0 | Short & Long | Short & Long | N.A. | [24,25,31,32] |
| ND5 | 0 | 0 | 1,779 | NE | 0.5 | 0.8 | 1.0 | N.D. | N.D. | N.A. | [9,25] |
| ND7 | 553 | 89 | 1,238 | 5’P/BS, 3’BSd | ~10 | N.D. | 1.0 | Short(E) | Short(UE) | 129 | [5,12] |
| ND8 | 259 | 46 | 574 | BSe | ~20 | N.D. | 1.0 | Short (E) & Long (E) | Short (E) | 70 | [5,13,24] |
| ND9 | 345 | 20 | 649 | BS | >1.0 | N.D. | 1.0 | Short (PE) & Long (E) | Short (PE) | 39 | [14] |
| RPS12 | 132 | 28 | 325 | BS | >1.0 | N.D. | 1.0 | Short(UE & E) & Long (E) | Short(UE & E) & Long (E) | 50 | [15] |
| Murf 2 | 26 | 4 | 1,111 | P/BS | ~1 | ~1 | ~1 | Short & Long | Short & Long | 1 | [27,33] |
| Murf 5 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.A. | N.D. |
| CR3 | 148 | 13 | 299 | BS | >1.0 | N.D. | 1.0 | N.D. | N.D. | 37 | [34] |
| CR4 | 325 | 40 | 567 | BS | 1.0 | N.D. | ~0 | Short (E) & Long (E) | Short (UE) | 41 | [35] |
aP, transcript is edited only in the procyclic (insect) developmental stage.
bP/BS, transcript is edited in both bloodstream and procyclic stages.
cNE, never edited, editing of these transcripts has not been reported.
dThe ND7 transcript is differentially edited in the procyclic and bloodstream stages.
eBS These transcripts are only fully edited in the bloodstream developmental stage [36].
fN.D. Values have not yet been determined.
gAll data comes from EATRO 164 procyclic gRNA transcriptome previously published [20].
hUE, unedited, the transcripts which carried these tails were typically unedited.
iE, edited, the transcripts carrying these tails were typically edited.
jN.A., Not applicable.
kCOII is a cis-edited transcript. Poly-A tails listed as short are between 10 and 50 nts long and tails listed as long are between 150 and 200 nts long.