Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 5;9:4105–4112. doi: 10.2147/OTT.S106907

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Song et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Expression and functions of GPR56 in NSCLC cell lines.

Notes: (A) GPR56 expression in adenocarcinoma cell line A549, squamous carcinoma cell line H520, and normal bronchial epithelial cell line HBE was demonstrated by Western blot (left) and ELISA (right). Western blot (B) and ELISA (C) results showed the efficiency of GPR56 overexpression and knockdown in A549 and H520 cell lines, respectively. With overexpression and knockdown, GPR56 was proven to be able to positively regulate cell proliferation (D, MTT assay) and invasion (E, Transwell assay) in cell lines A549 and H520. ^*P<0.05. The data represent mean ± standard deviation of three independent experiments.

Abbreviations: ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GPR56, G protein-coupled receptor 56; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium; NSCLC, non-small-cell lung carcinoma; SD, standard deviation.