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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Sci Transl Med. 2013 Aug 28;5(200):200ra115. doi: 10.1126/scitranslmed.3006373

Fig. 3. Effect of RbAp48-DN in the adult forebrain on object recognition memory.

Fig. 3

Data from novel object recognition task (mean ± SEM; one experiment). (A) Mice kept off doxycycline during the task. (a) Fifteen-minute training [DT: n = 11; control: n = 22 (tetO = 6, tTA = 8, WT = 8)]. DT (RbAp48-DN ON) mice performed worse than did controls during the 48-hour memory test (genotype × test effect: P = 0.0077, repeated-measures ANOVA; P = 0.0001, t test). (b) Ten-minute training [different groups of mice; DT: n = 12; control: n = 12 (tetO = 5, tTA = 4, WT = 3)]. DT mice performed worse than did controls in the 24-hour memory test (genotype × test effect: P = 0.0158, repeated-measures ANOVA; P = 0.0023, t test). (B) Animals kept on doxycycline during the task (mean ± SEM; one experiment). (a) Fifteen-minute training [DT: n = 10; control: n = 17 (tetO = 5, tTA = 5, WT = 7)]. (b) Ten-minute training [different groups; DT: n = 12; controls: n = 21 (tetO = 7, tTA = 7, WT = 7)]. DT on dox (RbAp48-DN OFF) and Control on dox displayed similar performance (P > 0.2, repeated-measures ANOVA). (C) Confocal images (30 μm) showing immunostaining against doublecortin (DCX) and Ki67 in the DG of 4-month-old RbAp48-DN–expressing (DT) and control mice. Graphs: Averaged data (±SEM). The numbers of DCX-and Ki67-expressing cells in DT and controls were similar (P > 0.5, ANOVA). DT: n = 24 (six mice; four slices per mouse) and controls: n = 24 [6 mice (tetO = 2, tTA = 2, WT = 2); four slices per mouse]. NeuN, marker of mature neurons. (D) Data from novel object recognition (mean ± SEM; one experiment). Young (3.5 months) and aged (15 months) WT mice. (a) Fifteen-minute training (n = 8 mice per age). Aged mice showed lower performance than did young mice in the 48-hour memory test (age × test effect: P = 0.0052, repeated-measures ANOVA; P = 0.0002, t test). (b) Ten-minute training (different group; n = 10 per age). Aged mice did not form 24-hour memory (age × test effect: P = 0.0021, repeated-measures ANOVA; P = 0.01, t test). *P = 0.01, **P = 0.0023, ***P < 0.0003 (A, B, and D). See table S3 for detailed statistics.