Chromatograms of 1000 nL injections of a small molecule mixture under isothermal (black line) and TASF (blue line) conditions with a gradient of 5−75% ACN over 12 min. Panel A illustrates all the solutes of the mixture whereas Panel B shows the early eluting peaks in more detail. The solutes in order of elution are acetanilide, methylparaben, acetophenone, ethylparaben, propiophenone, butyrophenone, benzophenone, valerophenone, hexanophenone, heptanophenone, and octanophenone. Panels are representative of n = 3 replicates.