Table I.
Plasmids used in the current study.
| Plasmid name | Vector | Insert(s) | Insertion site | Primers (5′-3′) |
|---|---|---|---|---|
| pGBKT7-FXR1 | pGBKT7 | FXR1 | EcoR I | ctagaattcatggcggacgtgacggtgctagaattcttatgaaacaccattc |
| pCMV-HA-FXR1 | pCMV-HA | FXR1 | EcoR I, XhoI | tatgaattcggatggcggagctgacggtggaggcgctcgagttatgaaacaccattcaggac |
| pCMV-Myc-CMAS | pCMV-Myc | BTF | EcoR I, XhoI | gtagaattccgatggactcggtggagcgtctcgagctatttttggcatgaat |
| pEGFP-N1-FXR1 | pEGFP-N1 | FXR1 | Xho I, EcoR I | tatctcgagctatggcggagctgacggtggaggcggaattcttatgaaacaccattcaggac |
| pDsRed-Monomer-N1-CMAS | pDsRed-Monomer | BTF | Xho I, EcoR I | tatctcgagctatggactcggtggagggcgaattcctatttttggcatgaat |
| pcDNA3.1(-)-FXR1 | pcDNA3.1(-) | FXR1 | EcoR I, XhoI | tatctcgagctatggcggagctgacggtggaggcggaattctatgaaacaccattcaggac |