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. 2016 Jun 7;14(2):1099–1106. doi: 10.3892/mmr.2016.5374

Figure 4.

Figure 4

Reverse transcription-polymerase chain reaction analysis of the mRNA expression of MMP2 and MMP9 in MDA-MB-231 cells, and the metaphysis and diaphysis of tibiae of BALB/c nu/nu mice. M, Tiangen DM:120227 DNA marker (50-500 bp) C, Human-β-actin (250 bp); C1, Mus-β-actin (154 bp); T1, target gene 1 (Human-MMP2) was positive in the MDA-MB-231 cells with a size of 112 bp). T2, target gene 2 (Human-MMP9) was positive in the MDA-MB-231 cells with a size of 139 bp). T3, target gene 3 (Mus-MMP2) was positive in the metaphysis of the tibiae of BALB/c nu/nu mice with a size of 140 bp). T4, target gene 4 (Mus-MMP9 was positive in the diaphysis of the tibiae of BALB/c nu/nu mice with a size of 229 bp. MMP, matrix metalloproteinase.