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Sequences of primers used for methylation analysis.
| Primer | Primer sequence | Product length (bp) |
|---|---|---|
| Methylated TGF-β3 primer | F: 5′-TATTTAATTAGATTGGAAAGGAGCG-3′ R: 5′-AACCGAAAATACCCTCTAACGA-3′ |
138 |
| Non-methylated TGF-β3 primer | F: 5′-ATTTAATTAGATTGGAAAGGAGTGG-3′ F: 5′-AAAAAACCAAAAATACCCTCTAACAA-3′ |
141 |
TGF-β3, transforming growth factor β3; F, forward; R, reverse.
