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. 2016 Jun 23;14(2):1465–1474. doi: 10.3892/mmr.2016.5435

Figure 3.

Figure 3

Downregulation of matriptase expression by RNA interference. (A) Microscopic examination of the infection efficiencies of different lentiviral-mediated SI constructs targeting matriptase in HO-8910PM cells (magnification, ×100). There was no signal observed in HO-8910PM-PBS. The infection efficiencies exceeded 80% at a MOI of 80. (B) Relative matriptase mRNA expression levels in HO-8910PM cells infected with different lentiviral particles at high MOI. Matriptase SI2 achieved the highest inhibition of matriptase mRNA expression, and little inhibition was observed in HO-8910PM-NC cells. The SI inhibited matriptase expression but not HAI-1 expression. The ratio of matriptase/HAI-1 was significantly reduced in the HO-8910PM cells treated with matriptase SI2. (C) Relative matriptase protein expression levels in HO-8910PM cells transfected with different lentiviral particles at high MOI. Protein quantification was normalized using β-actin. The highest silencing efficiency was achieved in HO-8910PM-SI2 cells using matriptase SI2. *P>0.05 vs. HO-8910PM; #P<0.05 vs. HO-8910PM. MOI, multiplicity of infection; HAI-1, hepatocyte growth factor activator inhibitor-1; NC, negative control; SI, small interfering RNA.