Figure 1.

Aspirin-mediated acetylation of glucose-6-phosphate dehydrogenase (G6PD) is greater in (A) HCT 116 cells compared with in (B) HT-29 cells. Subconfluent cells were left untreated or were treated with aspirin for 24 h, lysates were prepared, and equal amounts of protein were immunoprecipitated with rabbit agarose-conjugated anti-acetyl lysine antibody. Agarose-bound proteins were eluted and immunoblotted with anti-G6PD antibody. (C) HCT-116 and (D) HT-29 samples were immunoblotted with anti-G6PD antibody. The experiments were repeated three times.