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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Methods Mol Biol. 2012;849:347–359. doi: 10.1007/978-1-61779-551-0_23
10 cm plate 15 cm plate 12 × 15 cm
plates		 24 × 15 cm
plates
“Vector” 10.0 μg 22.5 μg 270 μg 540 μg
pGag/Pol 6.5 μg 14.6 μg 176 μg 352 μg
pRev 2.5 μg 5.6 μg 68 μg 136 μg
pVSV-G 3.5 μg 7.9 μg 95 μg 190 μg
H2O QS 450 μl QS 900 μl QS 12.15 ml QS 24.3 ml
CaCl2 (10×) 50 μl 100 μl 1.35 ml 2.7 ml
2× BBS 500 μl 1 ml 13.5 ml 27 ml