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. Author manuscript; available in PMC: 2016 Jul 11.
Published in final edited form as: Cell Metab. 2014 Dec 2;20(6):1059–1068. doi: 10.1016/j.cmet.2014.11.003

Figure 1. Genetic augmentation of NAD+ protects against NIHL.

Figure 1

WldS mice have reduced NIHL. WldS C57BL/6 transgenic animals (black) and littermate control C57BL/6 animals (grey) were exposed to 2 hr of 90 dB noise and then evaluated for changes in hearing 24 hr and 2 wks later by measuring auditory brainstem responses. WldS C57BL/6 animals showed marked protection as measured by both the levels of transient hearing loss at 24 hr and permanent hearing loss at 14 d in comparison to wild-type controls, most notably in the higher frequencies (16,000 and 32,000 Hz) typically affected by noise exposure. This demonstrates that genetically enhanced NAD+ biosynthesis can prevent hearing loss associated with noise exposure. Each point is the average threshold shift measured on a minimum of 7 mice per group. Statistical analysis was performed using a two-way ANOVA followed by a Bonferroni post-test (*P < 0.05, ***P < 0.001). Error bars represent SD.