Figure 4. WldS mice are protected against NIHL in a SIRT3-dependent fashion.
WT C57BL/6, WldSTg+/− C57BL/6, SIRT3−/− C57BL/6, and WldSTg+/−;SIRT3−/− C57BL/6 animals were tested for hearing loss just prior to a 2 hr 90 dB noise exposure, 24 hr after noise exposure, and 14 days after noise exposure. SIRT3 was necessary for WldS protection against NIHL for both transient and permanent hearing losses. Statistical analysis was performed using a two-way ANOVA followed by a Bonferroni post-test (***P < 0.001). Error bars represent SD.