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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Immunol. 2016 Jun 8;197(2):541–554. doi: 10.4049/jimmunol.1502529

Figure 3. OT-II transgene does not rescue positive selection in HDAC3-cKO mice.

Figure 3

(A) Examination of positive selection using Vβ5 and CD69 in OT-II and OT-II-HDAC3-cKO mice. Gating to identify stages of positive selection was performed as shown in Fig. 2a. (B) Examination of CD4 and CD8 surface expression in total thymocytes, and Vα2 and Vβ5 (OT-II) surface expression in TCRβ+ CD4SP and TCRβ+ CD8SP thymocytes from OT-II and OT-II-HDAC3-cKO mice. (C) Quantification of Vα2+ Vβ5+ CD4SP and Vα2+ Vβ5+ CD8SP thymocytes in OT-II and OT-II-HDAC3-cKO mice. (A–C) Data is representative of four mice per group from four independent experiments. (D) Quantification of Vα2+ Vβ5+ CD4 T cells and Vα2+ Vβ5+ CD8 T cells from the spleen of OT-II and OT-II-HDAC3-cKO mice. Data is representative of 3–4 mice per group from three independent experiments.