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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Immunol. 2016 Jun 8;197(2):541–554. doi: 10.4049/jimmunol.1502529

Figure 5. HDAC3-deficient thymocytes fail to down-regulate of RORγt during thymocyte positive selection.

Figure 5

(A) Intracellular expression of RORγt of pre-positive selection (TCRβloCD69lo), selecting (TCRβlo CD69+), and post-positive selection (TCRβ+CD69+) DP thymocytes. Plots are representative of at least ten mice per experimental group. (B) Intracellular expression of RORγt of DP and semi-mature CD4SP thymocytes (TCRβ+CD24+). Data is representative of at least ten mice per experimental group. (C) ChIP analysis of histone 3-acetylation (H3ac) at the RORC promoter of total thymocytes from WT and HDAC-cKO mice (numbers along x-axis correspond to primer sets in cartoon underneath plot; adapted from(26)). Results are presented as the fold-increase as compared to control ChIP with Ig alone [fold to Ig = (% input of H3ac)/(% input of Ig control)]. Data shown are the mean ± SEM of four mice per experimental group from three independent experiments.