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. 2016 Jun 28;5:e14470. doi: 10.7554/eLife.14470

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© 2016, Pérez Millán et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 6. — (A) Zeb2, Gli2 and Cdh1 mRNA levels in colonies from Prop1^df/+ and Prop1^df/df pituitaries treated with Zeb2 siRNA and negative control siRNA (control). Samples were normalized to GAPDH. qPCRs were done using at least three technical replicates. (B) Pictures of the colonies from Prop1^df/+ and Prop1^df/df pituitaries fixed and stained, show the different morphology of colonies between control siRNA and treated with Zeb2 siRNA.

DOI: http://dx.doi.org/10.7554/eLife.14470.010