Figure 9. PROP1 is a regulator of genes involved in EMT.

(A) HOMER analysis of new motif enrichment within PROP1 peaks. (B) Canonical Pathway Analysis of the putative PROP1 target genes found on the ChIP-Seq. (C) Streptavidin ChIP-Seq enrichment profiles. Enrichment peaks corresponding to Gli2, Notch2, Zeb2 and Cldn23 are indicated with asterisks. (D) Quantitative ChIP assay on candidate promoters. For each gene, primers were designed to amplify region where the PROP1 peak was detected. Note that enrichment of DNA fragments was specific to BirA, Prop1Tag cells, validating the specificity of ChIP-Seq. Experiments were done using three samples for each genotype (N = 3). Data are mean + SEM. OWA and * indicates p<0.05 relative to controls. Each ChIP experiment was repeated at least twice with similar results. (E) Table containing coordinates of the peaks found on each gene and the enriched motif found by HOMER. See also Figure 9—source data 1.

DOI: http://dx.doi.org/10.7554/eLife.14470.015