Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 8;5:e14814. doi: 10.7554/eLife.14814

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016, Mkhikian et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 1. — (A, B, D and E) T cells were activated with plate bound anti-CD3 for 24 (A and D) or 72 (B and E) hours. CD4⁺ (A and B) or CD8⁺ (D and E) cells were analyzed for CD69 expression (A and D) or 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution (B and E) by flow cytometry, gating on L-PHA^- cells where indicated. (C and F) T cells were analyzed for galectin-3 binding by flow cytometry, gating on CD4⁺ (C) or CD8⁺ (F) cells and L-PHA^- cells where indicated. Normalized geometric mean fluorescence intensity (MFI) is shown. Each mutant was normalized to its control. (G) Thymocytes and splenic T cells were analyzed for L-PHA and LEA binding by flow cytometry. (H) Cells were treated in culture with or without 500 nM SW for 72 hr followed by analysis of LEA binding by flow cytometry. Fold increase in LEA MFI of the SW treated sample compared to the untreated sample is presented. The red line marks one fold or no change. NS, not significant; *p<0.05; **p<0.01; ***p<0.001 (unpaired two-tailed t-test with Welch’s (C, F and H) and Bonferroni correction (C and F)). Data show one experiment representative of at least three independent experiments. Error bars indicate mean ± s.e.m.

DOI: http://dx.doi.org/10.7554/eLife.14814.003

Figure 1—figure supplement 1. — (A, B, D and E) T cells were activated with plate bound anti-CD3 for 24 (A and D) or 72 (B and E) hours. CD4⁺ (A and B) or CD8⁺ (D and E) cells were analyzed for CD69 expression (A and D) or 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution (B and E) by flow cytometry, gating on L-PHA^- cells where indicated. (C and F) T cells were analyzed for galectin-3 binding by flow cytometry, gating on CD4⁺ (C) or CD8⁺ (F) cells and L-PHA^- cells where indicated. Normalized geometric mean fluorescence intensity (MFI) is shown. Each mutant was normalized to its control. (G) Thymocytes and splenic T cells were analyzed for L-PHA and LEA binding by flow cytometry. (H) Cells were treated in culture with or without 500 nM SW for 72 hr followed by analysis of LEA binding by flow cytometry. Fold increase in LEA MFI of the SW treated sample compared to the untreated sample is presented. The red line marks one fold or no change. NS, not significant; *p<0.05; **p<0.01; ***p<0.001 (unpaired two-tailed t-test with Welch’s (C, F and H) and Bonferroni correction (C and F)). Data show one experiment representative of at least three independent experiments. Error bars indicate mean ± s.e.m.

DOI: http://dx.doi.org/10.7554/eLife.14814.003