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. Author manuscript; available in PMC: 2016 Aug 12.
Published in final edited form as: Oncogene. 2016 Jan 11;35(32):4179–4187. doi: 10.1038/onc.2015.483

Figure 3.

Figure 3

(a) Quantitative reverse transcriptase PCR (qRT–PCR) gene expression analysis of mouse normal small intestine of Apc+/+ Cftrfl/fl-Villin-Cre mice. Each gene sample was run in triplicate and gene expression was normalized to the expression of 18S. Data are presented as the mean fold change ±s.d. Each bar represents the mean and s.e. of multiple experiments that measured fold differences in the mRNA expression in proximal small intestine tissue isolated from adult (~100 days) littermate and gender matched pairs of Apc+/+ Cftrfl/fl-Villin-Cre and Cftr+/+ mice. mRNAs were isolated from 1 cm sections of the proximal small intestine from the same region for all mice. Villi were removed from the tissue prior to processing. Four replicates of each assay were performed for each matched pair of mRNAs and these sets of assays were repeated at least two times for each pair of mRNAs. At least two matched pairs of mRNAs were tested for each gene with most genes tested in at least three matched pairs of mRNAs. To be included in this figure genes met the flowing criteria: (1) the mean fold difference was at least 1.5; and (2) each gene showed a change in gene expression in the same direction in each matched pair of mRNAs. In all cases the direction of changes in gene expression confirmed microarray data. *P<0.05. (b) qRT–PCR gene expression analysis of mouse normal colon of Apc+/+ Cftrfl/fl-Villin-Cre mice. Samples were analyzed in triplicate and normalized to 18S ribosomal RNA. Data are presented as the mean fold change ± s.d. Each bar represents the mean and s.e. of multiple experiments that measured fold differences in the mRNA expression of whole colon tissue isolated from adult (~100 days), littermate and gender matched pairs of Apc+/+ Cftrfl/fl-Villin-Cre and Cftr+/+ mice. RNA was isolated from 1 cm sections from the same region of distal colon. Four replicates of each assay were performed for each matched pair of mRNAs and these sets of assays were repeated at least two times. At least two matched pairs of mRNAs were tested for each gene with most genes tested in at least three matched pairs of mRNAs. To be included in this figure genes met the flowing criteria: (1) the mean fold difference was at least 1.5; and (2) each gene showed a change in gene expression in the same direction in each matched pair of mRNAs. In all cases the direction in changes in gene expression confirmed microarray data. *P<0.05.