. Author manuscript; available in PMC: 2016 Aug 12.

Published in final edited form as: Oncogene. 2016 Jan 11;35(32):4179–4187. doi: 10.1038/onc.2015.483

Table 2.

Intestinal tumorigenesis in Apc^+/+ Cftr knockout mice

Class	N	Mean age (days)	Tumor penetrance (%)	Tumor multiplicity	Total tumors in colon	Total tumors in small intestine
Cftr^+/+	15	360	0	0	0	0
Cftr^fl/^fl-Villin-Cre	18	375	61	1.6	2	15

C57BL/6-Apc^+/+ Cftr^fl/fl-Villin-Cre⁺ mice and a contemporaneous control set of C57BL/6 Cftr wild-type mice, of roughly equal numbers by gender, were aged to ~ 1 year, then killed. Tumors were counted in formalin-fixed tissues from throughout the intestinal tract using a dissecting microscope. A subset of tumors was removed fresh frozen for RNA analysis and another subset was removed as formalin-fixed tissues for histopathological and immunohistochemical analysis. As has been observed before, no tumors were found in C57BL/6 Cftr wild-type control mice.