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. Author manuscript; available in PMC: 2016 Jul 12.
Published in final edited form as: Clin Immunol. 2016 Apr 27;168:6–15. doi: 10.1016/j.clim.2016.04.014

Fig. 5.

Fig. 5

Function of CD8+ T-cell memory subsets elicited after i.m. immunization with a PyCSP peptide mixed with 7DW8-5 and MPLA. Splenocytes were obtained from BALB/c mice after three doses of i.m. immunization with a PyCSP peptide alone, or with a PyCSP peptide mixed with 7DW8-5 and/or MPLA. Splenocytes from a group of non-immunized mice (n = 5) were used as a negative control. Then the percentage of Granzyme B+-CD11ahigh cells (A) IFN-γ+-cells (B) among CD8+ T-cells among naïve (CD44−CD62L+/−), central (CD44+CD62L+) and effector (CD44+CD62L−) subsets, respectively, were analyzed by FACS. In this figure, experiments were repeated twice. Statistical significance was displayed as ***, **, or *, if the p value is <0.001, <0.01, or <0.05, respectively. The abbreviation - n.s. - stands for “not significant” and was assigned if p ≥ 0.05.