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. Author manuscript; available in PMC: 2016 Aug 22.
Published in final edited form as: Oncogene. 2016 Jan 11;35(33):4335–4344. doi: 10.1038/onc.2015.496

Figure 4. FOXA1 acts as a pioneer factor that reprograms GATA2 cistrome.

Figure 4

A. Western blot analysis showing a slight decrease of GATA2 protein level following FOXA1 knockdown, especially in the presence of androgen.

B. Venn diagram showing greatly increased GTBS following FOXA1 knockdown. Control and FOXA1-knockdown (shFOXA1) LNCaP cells were hormone-deprived and subjected to GATA2 ChIP-seq.

C–D. Heatmap view (C) and average intensity plot (D) of GATA2 ChIP-seq read intensity around the three categories of GTBS identified in B.

E. FKHD and GATA motif intensity around the three categories of GTBS identified in B.

F–G. ChIP-PCR confirming increased GATA2 binding to target genes PSA (F) and TMPRSS2 (G) following FOXA1 knockdown. Control and FOXA1-knockdown LNCaP cells in the absence or presence of androgen were subjected to FOXA1 and GATA2 ChIP followed by qPCR analysis. Data shown are mean ± SEM in triplicate qPCR and is a representative of at least two independent experiments.