FIGURE 4.

Phylogenetic analysis of the Wolbachia clones obtained from the Dog and Phillips populations of T. putrescentiae. The phylogeny was inferred by a Bayesian analysis of 19 partial 16S rRNA gene sequences cloned from T. putrescentiae with the reference sequences from RDP, 242 known Wolbachia endosymbionts (Vandekerckhove et al., 1999; Hoy and Jeyaprakash, 2005; Haegeman et al., 2009; Russell et al., 2009; Augustinos et al., 2011; Glowska et al., 2015) and 27 type strains of the order Rickettsiales. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.05). Branch labels indicate Bayesian posterior probabilities and bootstrap support values for maximum likelihood analysis. The phylogram was rooted using Rhizobium oryzae sequence EU056823 as an outgroup.